Ultrasonication and RSM-based optimization of antioxidant activity, saccharide composition and fatty acids from Phoenix dactylifera L. Medjool date seeds influenced by ethanol

In response with the demand in date industry finding on sustainable solution for date seeds management and its bioactive rich constituent, current study envisaged the optimum condition for the ultrasound extraction of Phoenix dactylifera L. Medjool date seeds and its antioxidative activity by employ...

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Published inBulletin of the Chemical Society of Ethiopia Vol. 38; no. 3; pp. 547 - 562
Main Authors Thirubuvanesvari-Duraivelu, Pushpa, Gani, Siti Salwa Abd, Hassan, Masriana, Halmi, Mohd Izuan Effendi, Abutayeh, Reem Fawaz, Al-Najjar, Mohammad A.A, Abu-Odeh, Ala
Format Journal Article
LanguageEnglish
Published Chemical Society of Ethiopia 01.09.2024
Subjects
Acetaldehyde
Alcohol
Alcohol, Denatured
Analysis
Antioxidants
Fatty acids
Instrument industry
International economic relations
Seeds
Ultrasonic waves
United States
Malaysia
United Arab Emirates
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Summary:In response with the demand in date industry finding on sustainable solution for date seeds management and its bioactive rich constituent, current study envisaged the optimum condition for the ultrasound extraction of Phoenix dactylifera L. Medjool date seeds and its antioxidative activity by employing a three-level three-factor Box–Behnken design via response surface methodology (RSM). Ethanol (EtOH) concentration (50-80%), time (30-90 min) and temperature (40-70 °C) were the independent variables investigated for ABTS•+ scavenging antioxidant activity and subjected to analysis of variance (ANOVA). The optimum conditions for maximum antioxidant activity (60.93% ± 0.021) were achieved at 80% EtOH, 44 min and at 57 °C, where the effect of EtOH concentration were notably significant. The observed agreement between the experimental (60.93% ± 0.021) and predicted (60.35%) values indicated the employed model suitability while substantiates the successful implementation of RSM for optimizing extraction parameters. The optimized extract characterized through UPLC-QTOF/MS and GC-MS/MS, detailed the presence of saccharides (isomaltose, mannotriose and stachyose) and volatile compounds, namely 5 saturated fatty acids that encompassed within the 8.42% (w/w) of total fat obtained. This verifies the ability of the solvent mixture extracting fatty acids and saccharides even under high EtOH concentration. KEY WORDS: Date seeds, Response surface methodology (RSM), Antioxidant, Saccharide, GC-MS/MS, UPLC-QTOF/MS Bull. Chem. Soc. Ethiop. 2024, 38(3), 547-562.                                                                 DOI: https://dx.doi.org/10.4314/bcse.v38i3.1                                                            
ISSN:1011-3924
1726-801X
DOI:10.4314/bcse.v38i3.1