Estimation of cardiomyocyte transmembrane potential with potential-sensitive fluorescent probes

Transmembrane potentials on the plasma ([delta]Φ^sub 4^) and mitochondrial ([delta]Φ^sub m^) membrane of isolated rat cardiomyocytes were estimated using the potential-sensitive fluorescent probe DSM. The values were -93±4 and -196±11 mV, respectively. Sufan significantly decreased the reduction of...

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Published inBulletin of experimental biology and medicine Vol. 126; no. 5; pp. 1170 - 1172
Main Authors Khankoeva, A. I., Dukhanin, A. S., Galenko-Yaroshevskii, P. A.
Format Journal Article
LanguageEnglish
Published New York Springer Nature B.V 01.11.1998
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Summary:Transmembrane potentials on the plasma ([delta]Φ^sub 4^) and mitochondrial ([delta]Φ^sub m^) membrane of isolated rat cardiomyocytes were estimated using the potential-sensitive fluorescent probe DSM. The values were -93±4 and -196±11 mV, respectively. Sufan significantly decreased the reduction of [delta]Φ^sub m^ induced by chemical hypoxia. The effects of antiarrhythmic drugs on changes in [delta]Φ^sub p^ were studied using the fluorescent probe dis-C^sub 3^-(5). Lidocaine, novocainamide, richlocaine, and leocaine blocked depolarization of the myocyte plasma membrane induced by electrical stimulation and did not affect the [delta]Φ^sub m^.[PUBLICATION ABSTRACT]
ISSN:0007-4888
1573-8221
DOI:10.1007/BF02447265