Evaluation of the impact of decellularization and sterilization on tensile strength transgenic porcinedermal dressings

• Published in: Acta of bioengineering and biomechanics Vol. 21; no. 3; pp. 87 - 97
• Main Authors: Joszko, Kamil, Gzik-Zroska, Bożena, Kawlewska, Edyta, Klama-Baryła, Agnieszka, Suchoń, Sławomir, Burkacki, Michał, Wolański, Wojciech
• Format: Journal Article
• Language: English
• Published: Poland 2019
• Subjects: Acellular Dermis; Animals; Animals, Genetically Modified; Bandages; Elastic Modulus; Normal Distribution; Sterilization; Stress, Mechanical; Swine; Tensile Strength
• ISSN: 1509-409X
• DOI: 10.5277/ABB-01382-2019-02

The aim of this paper was to evaluate which method of acellularization and sterilization is optimal, in the meaning of which processes have the least impact on the deterioration of mechanical properties of porcine tissues used for xenogeneic applications. The static tensile probe was conducted for 80 skin specimens obtained from transgenic swine, which are used as a wound dressing for skin recipient. Obtained data were subsequently analyzed with the use of statistical methods. It was found that Young's modulus for the samples after the sterilization process for the dispase substance and the mixed method (SDS + trypsin) were statistically significantly changed. In the case of dispase, Young's modulus value before the sterilization process was 12.4 MPa and after the value increased to 28.0 MPa. For the mixed method (SDS + trypsin) before the sterilization process Young's modulus value was 5.6 MPa and after it was increased to 6.3 MPa. The mixed method (SDS + trypsin) had the slightest effect on changing the mechanical properties of the samples before and after the sterilization process. It was confirmed that different methods of acellularization and the process of sterilization have an influence on the change of mechanical properties of the skin of transgenic swine. In the authors' opinion, the mixed method (SDS + trypsin) should be recommended as the best one for the preparation of transgenic porcine dermal dressings because it ensures a smaller probability of dressing's damage during a surgical procedure.