miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer

• Published in: Cellular and Molecular Biology Vol. 63; no. 5; pp. 113 - 118
• Main Authors: Wei, Y-T., Guo, D-W., Hou, X-Z., Jiang, D-Q.
• Format: Journal Article
• Language: English
• Published: France 20.05.2017
• Subjects: Biomarkers, Tumor - metabolism; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell Proliferation - genetics; Cell Survival - genetics; Female; Forkhead Box Protein O1 - metabolism; Gene Expression Regulation, Neoplastic; Humans; MCF-7 Cells; MicroRNAs - genetics; MicroRNAs - metabolism; Cell proliferation; microRNA; Breast Cancer; FOXO1
• ISSN: 0145-5680; 1165-158X; 1165-158X
• DOI: 10.14715/cmb/2017.63.5.21

Breast cancer is the most commonly diagnosed cancer in women and a leading cause of cancer mortality. MicroRNAs (miRNAs) have been found to play a key role in proliferation, metastasis and invasion of cancer. In previous study, we found that miRNA-223 was significant expression inexosome derived from peripheral blood serum of breast cancer patients than in samples from control subjects, Therefor,the role ofmiRNA-223willbe researched in MCF-7 breast cancer cells.In this study, to explore the role of miRNA-223in influencing cell proliferation, metastasis and invasion of breast cancer, TargetScan tools (http://www.targetscan.org/vert_71/) was used to scan target genes of miRNA-223, and thenmiRNA expression, real time PCR, Western blotting andluciferase report assay were used to test regulates relationship of miRNA-223and its targets,cell viability and BrdU analysiswere used to test cell proliferation of MCF-7 breast cancer cells after expression miRNA-223inhibitor. Scanning targets of miRNA-223found FOXO1 was listed in targets content, and luciferase reporter assay was used to assess and confirm the binding sequence of 3"²untranslated region between FOXO1 and miRNA-223. Results showedthat miRNA-223inhibitorexpression increased protein expression level of FOXO1 in MCF-7 breast cancer cells,meanwhile, cell viability and BrdU analysis showed MCF-7 breast cancer cells were suppressed proliferation after up-regulation of FOXO1.In conclusion, we demonstrated that the miRNA-223can maintain cell proliferation of breast cancer cell through targeting FOXO 1, these results provide a new insight in tumor marker and potential therapeutic targets for breast cancer.