Turbidimetric Method for Quantitative Determination of Triton X-100 with Silicotungstic Acid

• Published in: Applied biochemistry and microbiology Vol. 38; no. 3; pp. 294 - 297
• Main Authors: Lagutina, L S, Sholts, K F
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.05.2002
• Subjects: Analytical chemistry; Calibration; Heterogeneity; Wavelengths
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1023/A:1015439912362

The formation of Triton X-100-silicotungstic acid complex was studied. Quantitative turbidimetric determination of the detergent based on this process was suggested. This method allows us to determine the complex formation at any wavelength in the range from 350 (ε^sub 350^ =15600 cm^sup -1^ M^sup -1^) to 600 nm (ε^sub 600^ = 9090 cm^sup -1^ M^sup -1^). The calibration curve for Triton X-100 recorded at 350 nm is linear in the concentration range of 0 to 30 μg/ml. A sigmoid calibration curve was observed at longer wavelengths. A linear fragment of the calibration curve recorded at 600 nm was found at a concentration of Triton X-100 of about 5 μg/ml. The complex nature of calibration curves can be explained by the heterogeneity of the complex dispersion.[PUBLICATION ABSTRACT]