In vitro evidence for a neurite growth-promoting activity in Trembler mouse serum

• Published in: International journal of developmental neuroscience Vol. 7; no. 3; p. 281
• Main Authors: Koenig, J, Hantaz-Ambroise, D, De La Porte, S, Do Thi, N A, Bourre, J M, La Chapelle, F, Koenig, H L
• Format: Journal Article
• Language: English
• Published: United States 1989
• Subjects: Animals; Cells, Cultured; Chondroitin Sulfate Proteoglycans - immunology; Chondroitin Sulfate Proteoglycans - physiology; Dendrites - drug effects; Dendrites - physiology; Floxuridine - pharmacology; Glycosaminoglycans - physiology; Heparan Sulfate Proteoglycans; Heparitin Sulfate - immunology; Heparitin Sulfate - physiology; Immune Sera; In Vitro Techniques; Laminin - immunology; Laminin - physiology; Mice; Mice, Neurologic Mutants - metabolism; Nerve Growth Factors - blood; Nerve Growth Factors - pharmacology; Proteoglycans - physiology; Spinal Cord - cytology; Spinal Cord - drug effects
• ISSN: 0736-5748
• DOI: 10.1016/0736-5748(89)90033-6

Basal lamina components, such as heparan sulfate proteoglycan (HSPG) and laminin play an important role in neuritic outgrowth for CNS and PNS neurons in culture. The mutant mouse 'Trembler' is characterized by hypomyelinization and production of an excess of basal lamina layers around Schwann cells in peripheral nerves. In order to analyse whether or not the serum of the mutant animals contains neurite outgrowth-promoting factors, we cultured rat spinal cord neurons in the presence of Trembler serum. Under these conditions, the outgrowth of neurites was increased approx. 2 times as compared to control serum. Trembler serum induces neuritic outgrowth characterized both by an increase in number of primary neurites emerging from the nerve cell body as well as by an increase in peripheral branching of neurites. To characterize the factors implicated in this increase we added antibodies directed against HSPG or laminin to the mutant serum. As a result, the increase in neuritic outgrowth was reduced or abolished in both cases. Trembler effect on neurite growth disappeared when the number of the non-neuronal cells was reduced, suggesting that the mutant serum did not act directly on neurons but by the intermediary action of non-neuronal cells.