Grape Pomace Polyphenols Reduce Acute Inflammatory Response Induced by Carrageenan in a Murine Model

• Published in: Chemistry & biodiversity Vol. 21; no. 7; pp. e202302065 - n/a
• Main Authors: Nery‐Flores, Sendar Daniel, Castro‐López, Cristina Marisela, Martínez‐Hernández, Lucía, García‐Chávez, Clara Victoria, Palomo‐Ligas, Lissethe, Ascacio‐Valdés, Juan Alberto, Flores‐Gallegos, Adriana Carolina, Campos‐Múzquiz, Lizeth Guadalupe, Rodríguez‐Herrera, Raúl
• Format: Journal Article
• Language: English
• Published: Switzerland Wiley Subscription Services, Inc 01.07.2024
• Subjects: Amberlite (trademark); Animal models; Animals; anti-inflammatory; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Carrageenan; Carrageenans; Cyclooxygenase 2 - metabolism; Cytokines; Cytokines - metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Edema; Edema - chemically induced; Edema - drug therapy; Grapes; Indomethacin; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - metabolism; Inflammatory response; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Male; Mice; natural products; Oxidative stress; paw edema; Peroxidase; Peroxidase - metabolism; Peroxidation; Polyphenols; Polyphenols - chemistry; Polyphenols - isolation & purification; Polyphenols - pharmacology; Prostaglandin endoperoxide synthase; Reagents; Ultrasonic imaging; Ultrasound; Vitis - chemistry; Vitis vinifera; Wines; anti-inflammatory; natural products; paw edema; polyphenols; Vitis vinifera
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202302065

Grape pomace (GP), a by‐product of wine production, contains bioactive polyphenols with potential health benefits. This study investigates the anti‐inflammatory properties of a polyphenolic fraction derived from GP, obtained by ultrasound‐microwave hybrid extraction and purified using ion‐exchange chromatography. In the inflammation model, mice were divided into six groups: intact, carrageenan, indomethacin, and three GP polyphenols treatment groups. Paw edema was induced by subplantar injection of carrageenan, and the GP polyphenols were administered intraperitoneally at doses of 10, 20, and 40 mg/kg. The anti‐inflammatory effect was evaluated by measuring paw volume, and expression of inflammatory markers: cyclooxygenase‐2 (COX‐2), myeloperoxidase (MPO), and cytokines (IL‐1β and IL‐6), along with lipid peroxidation levels. The GP polyphenols significantly reduced paw edema and expression levels of COX‐2, MPO, and cytokines in a dose‐dependent manner effect, with the highest dose showing the greatest reduction. Additionally, lipid peroxidation levels were also decreased by GP polyphenols treatment at doses of 10 and 20 mg/kg. These findings suggest that ultrasound‐microwave extraction combined with amberlite purification proved to be effective in obtaining a polyphenolic‐rich fraction from GP. Thus, GP polyphenols may serve as a natural anti‐inflammatory and antioxidant agent for treating inflammation and oxidative stress‐related diseases.