Development of microsatellite DNA markers for Indian Rat snake (Ptyas mucosa)

Thirteen microsatellite markers were developed for one of the vulnerable snake species Indian Rat snake (Ptyas mucosa) in Indian ecosystems that are now subject to much restoration efforts. All loci were polymorphic when tested across 25 individuals. The average number of alleles per locus varied fr...

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Published inForensic science international. Animals and environments Vol. 2; p. 100054
Main Authors Kothakota, Naga Jogayya, Haque, I.
Format Journal Article
LanguageEnglish
Published Elsevier 01.12.2022
Subjects
Conservation
DNA
forensic sciences
genetic structure
heterozygosity
loci
Microsatellite DNA markers
microsatellite repeats
population genetics
Ptyas mucosa
snakes
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Summary:Thirteen microsatellite markers were developed for one of the vulnerable snake species Indian Rat snake (Ptyas mucosa) in Indian ecosystems that are now subject to much restoration efforts. All loci were polymorphic when tested across 25 individuals. The average number of alleles per locus varied from 4 to 7 (mean 5.2). Observed and expected heterozygosity ranged from 0.76 to 1.00 and 0.59–0.94, respectively and the average polymorphic information content is 0.664 were identified demonstrating the utility of these markers for the investigation of the population genetic structure and diversity of this species.
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ISSN:2666-9374
2666-9374
DOI:10.1016/j.fsiae.2022.100054