Significance of sulfate- O-3-Xyl-MU synthesized by cultured human skin fibroblasts

Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-β- d-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xyl-MU derivatives. This Xyl-MU derivat...

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Published inInternational Congress series Vol. 1223; pp. 199 - 203
Main Authors Tazawa, Toshiyuki, Matsuya, Hideki, Kudo, Daisuke, Ishido, Keinosuke, Yoshihara, Syuichi, Sasaki, Mutsuo
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.12.2001
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Summary:Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-β- d-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xyl-MU derivatives. This Xyl-MU derivative was subjected to structural analysis and the results indicated that it was sulfate- O-3-Xyl-MU. This Xyl-MU derivative was also synthesized when Xyl-MU was incubated with [ 35S]PAPS. But when Gal-Xyl-MU and Gal-Gal-Xyl-MU were incubated with [ 35S]PAPS, incorporation of [ 35S]sulfate from [ 35S]PAPS into either galactose or xylose was not observed. When Xyl-MU or sulfate-Xyl-MU was incubated with UDP-[ 3H]Gal, incorporation of [ 3H]Gal into Xyl-MU was observed, but incorporation into sulfate-Xyl-MU was not. These results indicate that chain elongation from Xyl-MU is inhibited by sulfation of Xyl-MU, and that Xyl-MU sulfation is involved in the control of Xyl-MU-initiated glycosaminoglycan biosynthesis.
ISSN:0531-5131
1873-6157
DOI:10.1016/S0531-5131(01)00423-X