Activity of extracts and terpenoids from Tontelea micrantha (Mart. ex Schult.) A.C.Sm. (Celastraceae) against pathogenic bacteria

• Published in: Natural product research pp. 1 - 10
• Main Authors: Lopes, Ranieli Paiva, Ferreira, Fernanda Lopes, Faria de Sousa, Grasiely, Cruz Nizer, Waleska Stephanie da, Magalhães, Cintia Lopes de Brito, Ferreira, Jaqueline Maria Siqueira, Tótola, Antônio Helvécio, Duarte, Lucienir Pains, de Magalhães, José Carlos
• Format: Journal Article
• Language: English
• Published: England 08.02.2024
• Subjects: synergism; Vero cells toxicity; antibacterial effect; terpenoids; Tontelea micrantha
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2024.2309554

The pharmacological properties of plant extracts and phytochemicals, such as flavonoids and terpenoids, remain of great interest. In this work, the effect of extracts, friedelan-3,21-dione, and 3β- -D-glucosyl-sitosterol isolated from roots was evaluated against , , , and . The antibacterial activity was evaluated by the minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively), and the synergistic effect was assessed by the Checkerboard assay. Furthermore, the cytotoxicity of the plant-derived compounds against Vero cells was measured by the 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide (MTT) method. The biological effects of the isolated compounds were predicted using the PASS online software. The chloroform and hexane extracts of roots showed promising antibacterial effect, with MIC in the range of 4.8-78.0 µg/mL. Further analyses showed that these compounds do not affect the integrity of the membrane. The combination with streptomycin strongly reduced the MIC of this antibiotic and extracts. The extracts were highly toxic to Vero cells, and no cytotoxicity was detected for the two terpenoids isolated from them (i.e. friedelan-3,21-dione and 3β- -D-glucosyl-sitosterol; CC  > 1000 μg/mL). Therefore, extracts obtained from roots significantly inhibited bacterial growth and are considered promising agents against pathogenic bacteria. The cytotoxicity results were very relevant and can be tested in bioassays.