Generation of pancreatic secretory trypsin inhibitor gene knockout mice

Pancreatic secretory trypsin inhibitor (PSTI) is synthesized in the acinar cells of the pancreas and acts as a potent protease inhibitor that inhibits intrapancreatic activation of trypsin to prevent the occurrence of pancreatitis. Recently, we and other groups reported that some patients with chron...

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Bibliographic Details
Published inInternational Congress series Vol. 1255; pp. 197 - 198
Main Authors Omuraya, Masaki, Hirokawa, Kaoru, Araki, Masatake, Araki, Kimi, Hirota, Masahiko, Ogawa, Michio, Yamamura, Ken-ichi
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.08.2003
Subjects
Knockout mice
Pancreatitis
Protease inhibitor
PSTI
Trypsin
Knockout mice
Trypsin
PSTI
Protease inhibitor
Pancreatitis
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Summary:Pancreatic secretory trypsin inhibitor (PSTI) is synthesized in the acinar cells of the pancreas and acts as a potent protease inhibitor that inhibits intrapancreatic activation of trypsin to prevent the occurrence of pancreatitis. Recently, we and other groups reported that some patients with chronic pancreatitis had a point mutation in the PSTI gene. Genetic mutations in the PSTI gene seem to promote a predisposition to pancreatitis, possibly by lowering the threshold of pancreatitis caused by other factors. In order to prove this hypothesis, we generated PSTI knockout mice using cre/mutant lox system. Heterozygous offsprings of chimeras appeared entirely normal.
ISSN:0531-5131
1873-6157
DOI:10.1016/S0531-5131(03)00878-1