Phytochemical investigation of Nigrospora zimmermanii isolated from Poincianella pluviosa (Sibipiruna): metabolites characterisation and screening for anti-inflammatory activity

• Published in: Natural product research pp. 1 - 7
• Main Authors: Ferreira, Miller Santos, Katchborian-Neto, Albert, Nicácio, Karen de Jesus, Santos, Mario F C, Dias, Danielle F, Chagas-Paula, Daniela A, Soares, Marisi G
• Format: Journal Article
• Language: English
• Published: England 21.02.2024
• Subjects: natural products; Endophytic fungi; ITS sequencing; prostaglandin E2
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2024.2320733

Endophytic fungi residing symbiotically in plant tissues are promising sources of bioactive natural products. This study explored the anti-inflammatory potential of an endophytic fungus isolated from the Brazilian medicinal plant (Sibipiruna). The extract from the endophyte FPD13 exhibited potential anti-inflammatory effects by inhibiting prostaglandin E (PGE ) release by 75.22%. Phytochemical analysis using High-Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) enabled the isolation and identification of three compounds, including the macrolide Nigrosporolide, the phenyl-propanol Tyrosol, and the terpene Decarestrictine A. Morphological characteristics and Internal Transcribed Spacers region (ITS) sequencing classified fungus FPD13 as . The results reveal the anti-inflammatory potential and chemical diversity of endophytes, warranting further investigation into the bioactivity and structure elucidation of their bioactive metabolites.