S100A8/A9 in Myocardial Infarction

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1929; p. 739
• Main Authors: Sreejit, Gopalkrishna, Nooti, Sunil Kiran, Athmanathan, Baskaran, Nagareddy, Prabhakara Reddy
• Format: Journal Article
• Language: English
• Published: United States 2019
• Subjects: Animals; Biomarkers - blood; Biomarkers - metabolism; Blood - metabolism; Calgranulin A - genetics; Calgranulin A - metabolism; Calgranulin B - genetics; Calgranulin B - metabolism; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Fluorescent Antibody Technique; Humans; Mice; Monocytes - metabolism; Myocardial Infarction - genetics; Myocardial Infarction - metabolism; Myocardium - metabolism; Neutrophils - metabolism; Real-Time Polymerase Chain Reaction; Myocardial infarction; S100A8/A9; Mononuclear cells; Neutrophils
• DOI: 10.1007/978-1-4939-9030-6_46

S100A8/A9 represents a novel biomarker and therapeutic target in sterile inflammatory diseases. Among the various S100 proteins, S100A8 and S100A9 have been shown to be the most important of all the damage-associated molecular pattern (DAMP) proteins in sterile inflammatory conditions such as diabetes, cardiovascular disease, autoimmune disorders, etc. We present here methods to quantify S100A8/A9 expression in various tissues in mouse models of myocardial infarction (MI) using flow cytometry (FC), immunofluorescence, quantitative real-time polymerase chain reaction (q-RT-PCR), and enzyme-linked immunosorbent assays (ELISA).