S100A8/A9 in Myocardial Infarction

S100A8/A9 represents a novel biomarker and therapeutic target in sterile inflammatory diseases. Among the various S100 proteins, S100A8 and S100A9 have been shown to be the most important of all the damage-associated molecular pattern (DAMP) proteins in sterile inflammatory conditions such as diabet...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1929; p. 739
Main Authors Sreejit, Gopalkrishna, Nooti, Sunil Kiran, Athmanathan, Baskaran, Nagareddy, Prabhakara Reddy
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Animals
Biomarkers - blood
Biomarkers - metabolism
Blood - metabolism
Calgranulin A - genetics
Calgranulin A - metabolism
Calgranulin B - genetics
Calgranulin B - metabolism
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Antibody Technique
Humans
Mice
Monocytes - metabolism
Myocardial Infarction - genetics
Myocardial Infarction - metabolism
Myocardium - metabolism
Neutrophils - metabolism
Real-Time Polymerase Chain Reaction
Myocardial infarction
S100A8/A9
Mononuclear cells
Neutrophils
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Summary:S100A8/A9 represents a novel biomarker and therapeutic target in sterile inflammatory diseases. Among the various S100 proteins, S100A8 and S100A9 have been shown to be the most important of all the damage-associated molecular pattern (DAMP) proteins in sterile inflammatory conditions such as diabetes, cardiovascular disease, autoimmune disorders, etc. We present here methods to quantify S100A8/A9 expression in various tissues in mouse models of myocardial infarction (MI) using flow cytometry (FC), immunofluorescence, quantitative real-time polymerase chain reaction (q-RT-PCR), and enzyme-linked immunosorbent assays (ELISA).
ISSN:1940-6029
DOI:10.1007/978-1-4939-9030-6_46