Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2265; p. 213
• Main Authors: Beasley, Aaron B, Acheampong, Emmanuel, Lin, Weitao, Gray, Elin S
• Format: Journal Article
• Language: English
• Published: United States 2021
• Subjects: Antigens, Neoplasm - immunology; Antineoplastic Agents, Immunological - immunology; Biomarkers, Tumor - metabolism; Cell Culture Techniques - methods; Cell Line, Tumor; Humans; Immunomagnetic Separation - methods; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Melanoma - blood; Melanoma - metabolism; Melanoma - pathology; Neoplastic Cells, Circulating; Multi-marker; Circulating tumor cells (CTCs ); Immunomagnetic beads
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-1205-7_16

Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.