Parallelized Antibody Selection in Microtiter Plates

The most common in vitro technology to generate human antibodies is phage display. This technology is a key technology to select recombinant antibodies for the use as research tools, in diagnostic tests, and for the development of therapeutics.In this review, the high-throughput compatible selection...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1701; p. 273
Main Authors Russo, Giulio, Meier, Doris, Helmsing, Saskia, Wenzel, Esther, Oberle, Fabian, Frenzel, André, Hust, Michael
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Cloning, Molecular - methods
Gene Library
Humans
Peptide Library
Single-Chain Antibodies - genetics
Single-Chain Antibodies - immunology
Antibody selection
Phage display
Panning
Antibody
Monoclonal antibody screening
Single-chain fragment variable (scFv)
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Summary:The most common in vitro technology to generate human antibodies is phage display. This technology is a key technology to select recombinant antibodies for the use as research tools, in diagnostic tests, and for the development of therapeutics.In this review, the high-throughput compatible selection of antibodies (scFv) in microtiter plates is described. The given detailed protocols allow the antibody selection ("panning"), screening and identification of monoclonal antibodies in less than 1 week.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7447-4_14