Sources and regulation of nitric oxide synthesis in uterus smooth muscle cells

• Published in: Ukrainian biochemical journal Vol. 91; no. 4; pp. 33 - 40
• Main Authors: Danylovych, H. V., Danylovych, Yu. V., Bohach, T. V., Hurska, V. T., Kosterin, S. O.
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 01.08.2019
• Subjects: calcium; mitochondria; mtNOS; nitric oxide; uterus smooth muscles
• ISSN: 2409-4943; 2413-5003
• DOI: 10.15407/ubj91.04.033

It was proved that NO synthesis in isolated mitochondria of rat uterus smooth muscle depended on the entry of exogenous Ca2+ to mitochondria (inhibited by 1-10 mM Mg2+ in the absence of ATP and by 10 μM ruthenium red) and was suppressed by calmodulin antagonists (0.1-10 μM calmidazolium and 1-100 μM trifluoperazine). It was blocked by NG-nitro-L-arginine, a known antagonist of the constitutive NO-synthase, with a half-maximal inhibition effect at about 25 μM. Moderate deholesterinization of the plasma membrane of myocytes after processing with 0.01% digitonin was followed by increased nitric oxide biosynthesis by cells. The data obtained suggested that mitochondria and plasmalemma is a possible source of NO synthesis in uterine myocytes.