Stabilisation of Spent Wash by Polyhydroxybutyrate (PHB) Producing Microorganisms Isolated from Karad Region, Maharashtra

• Published in: Nature environment and pollution technology Vol. 22; no. 2; pp. 1041 - 1045
• Main Authors: Patil, G. V., Pathade, G. R.
• Format: Journal Article
• Language: English
• Published: Karad Technoscience Publications 01.06.2023
• Subjects: Alternative energy sources; Chemical oxygen demand; Citrobacter; Climate change; Distilleries; Effluent standards; Effluents; Flora; Hazardous areas; Industrial wastes; Klebsiella; Klebsiella grimontii; Liquid wastes; Microorganisms; phb producers, spent wash, cod, stabilization; Polyhydroxybutyrate; Polyhydroxybutyric acid; Quality standards; Reduction; Rhodococcus; Sewage; Sewage treatment; Soil water; Stabilization; Staphylococcus; Syrups & sweeteners; Treated sewage; Wastewater treatment; Watercourses
• ISSN: 2395-3454; 0972-6268; 2395-3454
• DOI: 10.46488/NEPT.2023.v22i02.052

In recent years, there has been a significant increase in the needs of the overgrowing population. Naturally, industrial belts increased worldwide to satisfy the variety and quantity of needs. While producing the products, a huge quantity of waste is generated and added continuously to the environment, causing hazardous effects on the flora and fauna. Spent wash is one of the most important types of industrial waste since it is the liquid waste that is left over after making alcohol and it pollutes the environment. Despite effluent quality standards, untreated or partially treated sewage frequently enters water courses or soils. It is always overloaded with organic and inorganic substances. The stabilization of the effluent by microorganisms is reflected in the form of COD reduction. The present study attempted to isolate the PHB-producing organisms and use them to stabilize the spent wash. The locally isolated, characterized, and identified 11 PHB-producing microorganisms from the Karad region of Maharashtra were used to stabilize spent wash. They include Candida orthopsilosis, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Klebsiella grimontii, Citrobacter fruendii, and members of Staphylococcus and Rhodococcus. All potential organisms were reported to stabilize the spent wash. The degree of stabilization was measured in terms of the percent reduction of COD and BOD. The % reduction was reported at 95.31% and 81.39% of COD and BOD, respectively, by Klebsiella grimontii, followed by 92.18% and 80.46% reductions of COD and BOD, respectively, by Staphylococcus. These organisms are promising in the treatment of spent wash.