Hemolytic Activity of Antimicrobial Peptides

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1548; p. 427
• Main Authors: Oddo, Alberto, Hansen, Paul R
• Format: Journal Article
• Language: English
• Published: United States 2017
• Subjects: Anti-Infective Agents - pharmacology; Antimicrobial Cationic Peptides - pharmacology; Dose-Response Relationship, Drug; Erythrocytes - drug effects; Hemolysis - drug effects; Humans; Melitten - pharmacology; Hemolysis; Melittin; Red blood cells; Antimicrobial peptides

For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control.