Hemolytic Activity of Antimicrobial Peptides

For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control.

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1548; p. 427
Main Authors Oddo, Alberto, Hansen, Paul R
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Anti-Infective Agents - pharmacology
Antimicrobial Cationic Peptides - pharmacology
Dose-Response Relationship, Drug
Erythrocytes - drug effects
Hemolysis - drug effects
Humans
Melitten - pharmacology
Hemolysis
Melittin
Red blood cells
Antimicrobial peptides
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Summary:For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6737-7_31