Hemolytic Activity of Antimicrobial Peptides
For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control.
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| Published in | Methods in molecular biology (Clifton, N.J.) Vol. 1548; p. 427 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
United States
2017
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| Subjects | |
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| Abstract | For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control. |
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| AbstractList | For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control. |
| Author | Hansen, Paul R Oddo, Alberto |
| Author_xml | – sequence: 1 givenname: Alberto surname: Oddo fullname: Oddo, Alberto organization: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark – sequence: 2 givenname: Paul R surname: Hansen fullname: Hansen, Paul R email: prh@sund.ku.dk organization: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark. prh@sund.ku.dk |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28013523$$D View this record in MEDLINE/PubMed |
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| Keywords | Hemolysis Melittin Red blood cells Antimicrobial peptides |
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| SubjectTerms | Anti-Infective Agents - pharmacology Antimicrobial Cationic Peptides - pharmacology Dose-Response Relationship, Drug Erythrocytes - drug effects Hemolysis - drug effects Humans Melitten - pharmacology |
| Title | Hemolytic Activity of Antimicrobial Peptides |
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