Partial characterization of the X chromosome of Diuraphis noxia (Hemiptera: Aphididae)

We present the first sequencing results after separation of the X chromosome of Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae), the largest known X chromosome described to date, using flow cytometry. The X chromosome of D. noxia is 0.1824 pg (1C) and an estimated 178.4 Mb (1C) in size. Mapping c...

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Bibliographic Details
Published inEntomological science Vol. 23; no. 1; pp. 86 - 94
Main Authors Steyn, Louis, Bierman, Anandi, Burger, N. Francois V., Botha, Anna‐Maria
Format Journal Article
LanguageEnglish
Published Kyoto, Japan John Wiley & Sons Australia, Ltd 01.03.2020
Blackwell Publishing Ltd
Subjects
Aphididae
Chromosomes
Diuraphis noxia
Entomology
Flow cytometry
flow sorting
Gene mapping
Genomes
Hemiptera
Illumina HiSeq 2000 system
Peptide mapping
Russian wheat aphid
sex chromosome
X chromosomes
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Summary:We present the first sequencing results after separation of the X chromosome of Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae), the largest known X chromosome described to date, using flow cytometry. The X chromosome of D. noxia is 0.1824 pg (1C) and an estimated 178.4 Mb (1C) in size. Mapping confirmed that the X chromosome contains 13,799 protein coding genes, but with a slight bias towards GC richness when compared to the complete D. noxia genome. Here we present the first sequencing results after separation of the X chromosome of Diuraphis noxia (Kurdjumov, Hemiptera: Aphididae), the largest known X chromosomes described to date, using flow cytometry. The X chromosome of D. noxia is 0.18 pg (1C) and an estimated 178 Mb (1C) in size. Mapping confirmed that the X chromosome contains 13,799 protein coding genes, but with a slight bias towards GC richness when compared to the complete D. noxia genome.
ISSN:1343-8786
1479-8298
DOI:10.1111/ens.12401