Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules

The discovery of antibodies that bind to targets with high affinity is now a routine exercise. However, it is still challenging to screen for candidates that, in addition to having excellent biological properties, also have optimal biophysical characteristics. Here, we describe a simple HPLC-based s...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1575; p. 189
Main Authors Kohli, Neeraj, Geddie, Melissa L
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Adsorption
Antibodies - chemistry
Antibodies - isolation & purification
Antibodies, Monoclonal
Antibody Specificity
Chromatography, Gel
Chromatography, High Pressure Liquid
Protein Stability
Developability
Size exclusion chromatography
Colloidal stability
Zenix column
Standup monolayer adsorption chromatography
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Summary:The discovery of antibodies that bind to targets with high affinity is now a routine exercise. However, it is still challenging to screen for candidates that, in addition to having excellent biological properties, also have optimal biophysical characteristics. Here, we describe a simple HPLC-based screening method to assess for developability factors earlier in the discovery process.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6857-2_11