Environmentally dependent interactions shape patterns in gene content across natural microbiomes

• Published in: Nature microbiology Vol. 9; no. 8; pp. 2022 - 2037
• Main Authors: Crocker, Kyle, Lee, Kiseok Keith, Chakraverti-Wuerthwein, Milena, Li, Zeqian, Tikhonov, Mikhail, Mani, Madhav, Gowda, Karna, Kuehn, Seppe
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.08.2024; Nature Publishing Group
• Subjects: 631/158/853; 631/57; 639/766/747; Acidic soils; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biomedical and Life Sciences; Community composition; Denitrification; Enrichment; Environmental conditions; Genes; Genotype; Genotypes; Hydrogen-Ion Concentration; Infectious Diseases; Life Sciences; Medical Microbiology; Metabolism; Metagenomics; Microbiology; Microbiomes; Microbiota - genetics; NAR gene; Nitrogen; Oceans; Parasitology; pH effects; Phenotypes; Physics; Soil - chemistry; Soil Microbiology; Soil microorganisms; Surveys; Taxonomy; Virology
• ISSN: 2058-5276; 2058-5276
• DOI: 10.1038/s41564-024-01752-4

Sequencing surveys of microbial communities in hosts, oceans and soils have revealed ubiquitous patterns linking community composition to environmental conditions. While metabolic capabilities restrict the environments suitable for growth, the influence of ecological interactions on patterns observed in natural microbiomes remains uncertain. Here we use denitrification as a model system to demonstrate how metagenomic patterns in soil microbiomes can emerge from pH-dependent interactions. In an analysis of a global soil sequencing survey, we find that the abundances of two genotypes trade off with pH; nar gene abundances increase while nap abundances decrease with declining pH. We then show that in acidic conditions strains possessing nar fail to grow in isolation but are enriched in the community due to an ecological interaction with nap genotypes. Our study provides a road map for dissecting how associations between environmental variables and gene abundances arise from environmentally modulated community interactions. Global soil sequencing data and enrichment experiments show trade-offs in denitrification phenotypes driven by pH, underscoring how the environment shapes ecological interactions and gene content.