Optical and topographic characteristics of silver films deposited from a colloidal solution on polyelectrolytes for IgG-FITC fluorescence analysis

The optimization of the fluorescence enhancement factor of the IgG-FITC conjugate as an immunofluorescent marker, depending on the optical and topographic parameters of the colloidal silver film on the surface of a polystyrene plate for immunoassay, was carried out for the first time, and the factor...

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Published inZeitschrift für physikalische Chemie (Neue Folge) Vol. 236; no. 11; pp. 1603 - 1615
Main Authors Kulakovich, Olga, Scherbovich, Anastasia, Koktysh, Irina, Melnikova, Yanina, Ramanenka, Andrei, Gaponenko, Sergey, Maskevich, Sergey
Format Journal Article
LanguageEnglish
Published Munich De Gruyter 16.12.2022
Walter de Gruyter GmbH
Subjects
Fluorescence
IgG-FITC
Immunoassay
immunofluorescence analysis
microplates
Nanoparticles
Optical density
Optimization
Parameters
plasmon-enhanced fluorescence
polyelectrolyte
Polyelectrolytes
Polystyrene resins
Silver
silver nanoparticles
Solid phases
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Summary:The optimization of the fluorescence enhancement factor of the IgG-FITC conjugate as an immunofluorescent marker, depending on the optical and topographic parameters of the colloidal silver film on the surface of a polystyrene plate for immunoassay, was carried out for the first time, and the factors effecting the enhancement were identified. By means of time-resolved spectroscopy as well as by detection the relative concentrations of IgG-FITC adsorbed on the solid phase with enzyme-linked immunoassay, it was shown that fluorescence enhancement in the presence of silver nanoparticles is a resonance process associated with plasmon effects. The most important parameters correlating with the fluorescence enhancement factor are the optical density value at the wavelength of excitation and emission of the fluorophore. The maximum enhancement factor of 10.2 times was obtained for Ag films with the highest optical density.
ISSN:0942-9352
2196-7156
DOI:10.1515/zpch-2021-3110