Removal of Double-Stranded RNA Contaminants During Template-Directed Synthesis of mRNA
The removal of double-stranded RNA (dsRNA) contaminants during in vitro mRNA synthesis is one of the technological problems to be solved. Apparently, these contaminants are the result of the T7 RNA polymerase side activity. In this study, we used a modified method of mRNA purification based on the s...
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Published in | Bulletin of experimental biology and medicine Vol. 176; no. 6; pp. 751 - 755 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.04.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The removal of double-stranded RNA (dsRNA) contaminants during
in vitro
mRNA synthesis is one of the technological problems to be solved. Apparently, these contaminants are the result of the T7 RNA polymerase side activity. In this study, we used a modified method of mRNA purification based on the selective binding of dsRNA to cellulose in ethanol-containing buffer. It was shown both
in vivo
and
in vitro
that the cellulose-purified mRNA preparation leads neither to activation of the lymphocyte inflammatory marker CD69 nor to increased release of IFNα in mice, and does not contain impurities detectable by antibodies to dsRNA. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-4888 1573-8221 1573-8221 |
DOI: | 10.1007/s10517-024-06102-2 |