The Diagnostic Value of Antibodies Against Citrullinated or Carbamylated Fibrinogen in Rheumatoid Arthritis

• Published in: Clinical laboratory (Heidelberg) Vol. 66; no. 5
• Main Authors: Wang, Zhenni, Xue, Luan, Xie, Jun, Zhu, Naishuo
• Format: Journal Article
• Language: English
• Published: Germany 01.01.2020
• Subjects: Aged; Arthritis, Rheumatoid - diagnosis; Autoantibodies - blood; Autoantigens - chemistry; Autoantigens - immunology; Citrullination; Enzyme-Linked Immunosorbent Assay; Female; Fibrinogen - chemistry; Fibrinogen - immunology; Humans; Male; Middle Aged; Peptides - chemistry; Peptides - immunology; Protein Carbamylation; Sensitivity and Specificity
• ISSN: 1433-6510
• DOI: 10.7754/Clin.Lab.2020.191256

This study aimed to evaluate the overall diagnostic value of citrullinated or carbamylated fibrinogen antibodies in patients with rheumatoid arthritis (RA). Serum samples collected from 114 patients with established RA, 143 patients with non-RA diseases, and 200 healthy controls were tested by ELISA for citrullinated fibrinogen (Cit-fib), carbamylated fibrinogen (Ca-fib), and chimeric fibrinogen a/b chain citrullinated peptides (CFABCP). Diagnostic indexes and correlations with titers were calculated, cross reactivities of Cit-fib, Ca-fib, and CFABCP were assessed by competition experiments. With a cutoff ensuring 98% specificity for RA patients versus healthy controls, the sensitivities of Cit-fib and Ca-fib are 66.67% and 24.6%, respectively, while the sensitivity of CFABCP was 74.56%. Cit-fib, Ca-fib, and CFABCP can inhibit reciprocally in competition experiments. As for non-RA patients, the positive rate of Ca-fib was higher than that of Cit-fib and CFABCP. Citrullination and carbamylation of fibrinogen both have a role in RA diagnosing, but citrullination is better. The recombination of peptides, CFABCP, has high specificity and considerable sensitivity for diagnosis for RA patients.