In silico studies and development of a protein-based electrochemical sensor for selective and sensitive detection of aflatoxin B1

• Published in: Mikrochimica acta (1966) Vol. 191; no. 7; p. 426
• Main Authors: Ulaganambi, Megala, S., Lokesh Kumar, Kumar, Sanjit, Tetala, Kishore K. R.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.07.2024; Springer Nature B.V
• Subjects: Aflatoxin B1 - analysis; Aflatoxin B1 - immunology; Aflatoxins; Analytical Chemistry; Arachis - chemistry; Biosensing Techniques - methods; Carbon nanotubes; Characterization and Evaluation of Materials; Chemical sensors; Chemistry; Chemistry and Materials Science; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrodes; Glassy carbon; Humans; IgG antibody; Immunoglobulin G; Immunosensors; Infrared spectroscopy; Kinases; Limit of Detection; Microengineering; Molecular Docking Simulation; Nanochemistry; Nanocomposites; Nanotechnology; Nanotubes, Carbon - chemistry; Original Paper; Proteins; Proto-Oncogene Mas; Reductases; Carbon nanotube-nanofiber; Human serum immunoglobulin G; Modified glassy carbon electrode; AFB1; Electrochemical sensor; Differential pulse voltammetry
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-024-06495-x

Proteins from different species have been docked with aflatoxin B1 (AFB1) and identified 3 proteins (prostaglandin-E(2)9-reductase from Oryctolagus uniculus , proto-oncogene serine/threonine-protein kinase Pim-1 and human immunoglobulin G (hIgG)) as potential candidates to develop an electrochemical sensor. Fluorescence spectroscopy experiments have confirmed the interaction of hIgG with AFB1 with an affinity constant of 4.6 × 10 5 M −1 . As a proof-of-concept, hIgG was immobilized on carbon nanocomposite (carbon nanotube-nanofiber, CNT-F)-coated glassy carbon electrode (GCE). FT-IR spectra, HR-TEM and BCA assay have confirmed successful immobilization of hIgG on the electrode (hIgG@CNT-F/GCE). The preparation of this protein electrochemical sensor requires only 1 h 36 min, which is fast as compared with preparing an electro immunosensor. hIgG@CNT-F/GCE has displayed an excellent AFB1 limit of detection (0.1 ng/mL), commendable selectivity in the presence of two other mycotoxins (ochratoxin A and patulin) and the detection of  AFB1 in spiked peanuts and corn samples. Graphical abstract