Involvement of interleukin-10 (IL-10) and viral IL-6 in the spontaneous growth of Kaposi's sarcoma herpesvirus-associated infected primary effusion lymphoma cells

• Published in: Blood Vol. 94; no. 8; pp. 2871 - 2879
• Main Authors: JONES, K. D, AOKI, Y, YUAN CHANG, MOORE, P. S, YARCHOAN, R, TOSATO, G
• Format: Journal Article
• Language: English
• Published: Washington, DC The Americain Society of Hematology 15.10.1999
• Subjects: AIDS/HIV; Antibodies, Monoclonal - pharmacology; Autocrine Communication - drug effects; Autocrine Communication - physiology; Biological and medical sciences; Cell Division; Culture Media, Conditioned - pharmacology; Hematologic and hematopoietic diseases; Herpesvirus 8, Human - physiology; Humans; Immune Sera; Interleukin-10 - antagonists & inhibitors; Interleukin-10 - immunology; Interleukin-10 - metabolism; Interleukin-10 - physiology; Interleukin-6 - antagonists & inhibitors; Interleukin-6 - immunology; Interleukin-6 - metabolism; Interleukin-6 - physiology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma, B-Cell - pathology; Lymphoma, B-Cell - virology; Medical sciences; Receptors, Interleukin - antagonists & inhibitors; Receptors, Interleukin - immunology; Receptors, Interleukin-10; Tumor Cells, Cultured; Viral Proteins - antagonists & inhibitors; Viral Proteins - immunology; Viral Proteins - metabolism; Viral Proteins - physiology; Human; Cell proliferation; Skin disease; Pathogenesis; Herpesviridae; Alphaherpesvirinae; Cytokine; Malignant hemopathy; Malignant tumor; In vitro; Lymphoma; Virus; Interleukin 6; Kaposi sarcoma; Lymphoproliferative syndrome; Malignant effusion; Interleukin 10; Established cell line; Herpesvirus hominis; Tumor cell; Growth factor
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V94.8.2871

Primary effusion lymphoma (PEL) is a distinct type of lymphoma associated with Kaposi's sarcoma-associated herpesvirus (KSHV) infection. To determine the factors responsible for the unrestrained proliferation of PEL, we have studied the growth factor requirements of the PEL-derived BCBL-1 and BC-1 cell lines. Both cell lines were found to be autocrine growth factor dependent and to release human interleukin-6 (IL-6), viral IL-6 (vIL-6), and human IL-10 in the culture supernatant. To establish whether these cytokines contribute to autocrine growth, neutralizing antibodies against human IL-6, vIL-6, human IL-10, and soluble IL-10 receptor were used. These experiments showed that human IL-10 and, to a lesser degree, vIL-6 serve as autocrine growth factors for BCBL-1 and BC-1 cells. Thus, human IL-10 and vIL-6 are growth factors released and used by PEL cells for autonomous proliferation and may be critical to the development and progression of PEL.