Prevalence of cagA, cagM, vacA and oipA genes in isolates of Helicobacter pylori obtained from hospital patients in Northeast Brazil
Helicobacter pylori is a major cause of gastrointestinal disorders such as chronic gastritis, peptic ulcers, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. It is estimated that around half of the world’s population is infected with this pathogen, with underdeveloped countries...
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Published in | Brazilian journal of microbiology Vol. 55; no. 3; pp. 2631 - 2641 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.09.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Helicobacter pylori
is a major cause of gastrointestinal disorders such as chronic gastritis, peptic ulcers, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. It is estimated that around half of the world’s population is infected with this pathogen, with underdeveloped countries reporting the highest frequencies. The genes
cag
A,
cag
M,
vac
A, and
oip
A are some of the most important virulence factors of
H. pylori
; however, there are no recent studies from Recife-PE demonstrating their frequency, and their relationship with severe gastric modifications. This work aims to use qualitative PCR to detect the virulence genes
cag
A,
cag
M,
vac
A, and
oip
A in
H. pylori
isolates obtained from patients in a public hospital in Recife (PE). We collected samples from the stomach’s body and antrum of 147 patients, from which 71 (48%) tested positive for
H. pylori
. Among positive samples, the most frequently infected gender was female (44/71, 62%), and the most frequently infected age group was those above the age of 46 (31/71, 44%). Histological examination of
H. pylori
-positive samples revealed alterations other than chronic gastritis, including metaplasia and atrophy. The frequency of
cag
A,
cag
M, and
oip
A genes were identified in 84%, 56%, and 69% of the samples tested, respectively, as well as the
vac
A
-
s1m
1
allelic combination (77%). However, there was no statistically significant variation in the occurrence of these genes, therefore they cannot be considered unique markers of severity in our setting. New research with larger samples and investigations of other genetic markers can aid uncover local risk factors and lead to a better understanding of
H. pylori
’s pathogenesis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1517-8382 1678-4405 1678-4405 |
DOI: | 10.1007/s42770-024-01380-4 |