Mapping of a novel locus Ra conferring extreme resistance against potato virus A in cultivated potato (Solanum tuberosum L.)

• Published in: Theoretical and applied genetics Vol. 137; no. 8; p. 198
• Main Authors: Huang, Wei, Zheng, Jie, Nie, Bihua, Li, Jiana, Chen, Ruhao, Nie, Xianzhou, Tu, Zhen, Gardner, Kyle, Chen, Jiaru, Yang, Manhua, Li, Jingcai, Dong, Jianke, Ma, Hui, Song, Botao
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2024; Springer Nature B.V
• Subjects: Agriculture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome 4; Chromosome deletion; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Crop yield; Cultivars; Disease resistance; Disease Resistance - genetics; Gene mapping; Genetic Markers; Genotype; Genotypes; INDEL Mutation; Life Sciences; Marker-assisted selection; Next-generation sequencing; Original Article; Phenotype; Phenotypes; Plant Biochemistry; Plant Breeding; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - virology; Plant Genetics and Genomics; Plant virus diseases; Plant viruses; Potatoes; Potyvirus - pathogenicity; Solanum tuberosum; Solanum tuberosum - genetics; Solanum tuberosum - virology; Tetraploidy; Viruses
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-024-04705-x

Key message The Ra extreme resistance against potato virus A was mapped to the upper of chromosome 4 in tetraploid potato. Potato virus A (PVA) is one of the major viruses affecting potato worldwide and can cause serious disease symptoms and yield losses. Previously, we determined that potato cultivar Barbara harbors Ry sto (genotype: Ryryryry ) and Ra (genotype: Rararara ) that each independently confer extreme resistance to PVA. In this study, employing a combination of next-generation sequencing and bulked-segregant analysis, we further located this novel Ra on chromosome 4 using a tetraploid BC 1 potato population derived from a Ry -free progeny ( Rararararyryryry ) of Barbara ( RarararaRyryryry ) × F58050 ( rararararyryryry ). Using 29 insertion–deletion (InDel) markers spanning chromosome 4, Ra was delimited by the InDel markers M8-83 and M10-8 within a genetic interval of 1.46 cM, corresponding to a 1.86-Mb genomic region in the potato DM reference genome. The InDel marker M10-8, which is closely linked with the resistance against PVA in the Ry -free segregating populations, was then used to screen 43 selected Ry sto -free tetraploid potato breeding clones. The phenotype to PVA was significantly correlated with the present/absent of the marker, albeit with a 9.3% false positive rate and a 14.0% false negative rate. These findings are of importance in furthering the cloning of Ra and employing the marker-assisted selection for PVA resistance.