Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection

• Published in: Bulletin of experimental biology and medicine Vol. 176; no. 6; pp. 776 - 780
• Main Authors: Kisakov, D. N., Kisakova, L. A., Sharabrin, S. V., Yakovlev, V. A., Tigeeva, E. V., Borgoyakova, M. B., Starostina, E. V., Zaikovskaya, A. V., Rudometov, A. P., Rudometova, N. B., Karpenko, L. I., Ilyichev, A. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2024; Springer Nature B.V
• Subjects: Antibodies; Antigens; Bioengineering; Biomedical and Life Sciences; Biomedicine; Cell Biology; COVID-19 vaccines; Genes; Immune response; Immunization; Injection; Internal Medicine; Laboratory Medicine; Lipids; Lymphocytes T; mRNA; mRNA vaccines; Nanoparticles; Pathology; Peptides; Proteins; Research centers; Severe acute respiratory syndrome coronavirus 2; Software; Virology; Viruses; jet injection; mRNA vaccines; delivery methods; receptor-binding domain of the S protein of the SARS-CoV-2 virus
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-024-06107-x

We studied a needle-free jet injection delivery of an experimental mRNA vaccine encoding the receptor-binding domain of the SARS-CoV-2 S protein (mRNA-RBD). Immunization of BALB/c mice with mRNA-RBD by a needle-free jet injector induced high levels of antibodies with virus-neutralizing activity and a virus-specific T-cell response. The immune response was low in the group of mice that received intramuscular injection of mRNA-RBD. The effectiveness of this simple and safe method of mRNA delivering has been demonstrated. Thus, jet injection of mRNA vaccine can be a good alternative to lipid nanoparticles.