CRISPR-Cpf1 system and its applications in animal genome editing

• Published in: Molecular genetics and genomics : MGG Vol. 299; no. 1; p. 75
• Main Authors: Han, Yawei, Jia, Zisen, Xu, Keli, Li, Yangyang, Lu, Suxiang, Guan, Lihong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2024; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Bacteria; Bacterial Proteins; Biochemistry; Biomedical and Life Sciences; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; CRISPR; CRISPR-Associated Proteins; CRISPR-Cas Systems - genetics; Crystal structure; Endodeoxyribonucleases; Endonuclease; Gene Editing - methods; Gene therapy; Genetic engineering; Genetic Therapy - methods; Genome - genetics; Genome editing; Genomes; Genomics; Human Genetics; Humans; Life Sciences; Microbial Genetics and Genomics; Plant Genetics and Genomics; Proteins; Review; RNA editing; Stem cells; Animal model; CRISPR-Cpf1; Genome editing; Gene therapy
• ISSN: 1617-4615; 1617-4623; 1617-4623
• DOI: 10.1007/s00438-024-02166-x

The clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein (Cas) system is a gene editing technology guided by RNA endonuclease. The CRISPR-Cas12a (also known as CRISPR-Cpf1) system is extensively utilized in genome editing research due to its accuracy and high efficiency. In this paper, we primarily focus on the application of CRISPR-Cpf1 technology in the construction of disease models and gene therapy. Firstly, the structure and mechanism of the CRISPR-Cas system are introduced. Secondly, the similarities and differences between CRISPR-Cpf1 and CRISPR-Cas9 technologies are compared. Thirdly, the main focus is on the application of the CRISPR-Cpf1 system in cell and animal genome editing. Finally, the challenges faced by CRISPR-Cpf1 technology and corresponding strategies are analyzed. Although CRISPR-Cpf1 technology has certain off-target effects, it can effectively and accurately edit cell and animal genomes, and has significant advantages in the preclinical research.