In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus
The inflammasome is a multimeric protein complex that plays a vital role in the defence against pathogens and is therefore considered an essential component of the innate immune system. In this study, the expression patterns of inflammasome genes ( NLRC3 , ASC , and CAS-1 ), antiviral genes ( IFNγ a...
Saved in:
Published in | Archives of virology Vol. 169; no. 7; p. 148 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Vienna
Springer Vienna
01.07.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The inflammasome is a multimeric protein complex that plays a vital role in the defence against pathogens and is therefore considered an essential component of the innate immune system. In this study, the expression patterns of inflammasome genes (
NLRC3
,
ASC
, and
CAS-1
), antiviral genes (
IFNγ
and
MX
), and immune genes (
IL-1β
and
IL-18
) were analysed in
Oreochromis niloticus
liver (ONIL) cells following stimulation with the bacterial ligands peptidoglycan (PGN) and lipopolysaccharide (LPS) and infection with TiLV. The cells were stimulated with PGN and LPS at concentrations of 10, 25, and 50 µg/ml. For viral infection, 10
6
TCID
50
of TiLV per ml was used. After LPS stimulation, all seven genes were found to be expressed at specific time points at each of the three doses tested. However, at even higher doses of LPS,
NLRC3
levels decreased. Following TiLV infection, all of the genes showed significant upregulation, especially at early time points. However, the gene expression pattern was found to be unique in PGN-treated cells. For instance,
NLRC3
and
ASC
did not show any response to PGN stimulation, and the expression of
IFNγ
was downregulated at 25 and 50 µg of PGN per ml.
CAS-1
and
IL-18
expression was downregulated at 25 µg of PGN per ml. At a higher dose (50 µg/ml), IL-1β showed downregulation. Overall, our results indicate that these genes are involved in the immune response to viral and bacterial infection and that the degree of response is ligand- and dose-dependent. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0304-8608 1432-8798 1432-8798 |
DOI: | 10.1007/s00705-024-06077-5 |