Correlation of Blood and Intestinal Mucosa miR-34a Expressions with Disease Severity in Ulcerative Colitis Patients

• Published in: Clinical laboratory (Heidelberg) Vol. 70; no. 5
• Main Authors: Li, Yi-Lan, Cai, Chu-Quan, Shi, Xiao-Lin, Xu, Xian-Feng, Guo, Li-Jia
• Format: Journal Article
• Language: English
• Published: Germany 01.01.2024
• Subjects: Biomarkers - blood; Case-Control Studies; Colitis, Ulcerative - blood; Colitis, Ulcerative - diagnosis; Colitis, Ulcerative - genetics; Colitis, Ulcerative - metabolism; Female; Humans; Interleukin-23 - blood; Interleukin-23 - genetics; Intestinal Mucosa - metabolism; Male; MicroRNAs - blood; MicroRNAs - genetics; RNA, Messenger - blood; RNA, Messenger - genetics; RNA, Messenger - metabolism; ROC Curve; Severity of Illness Index; STAT3 Transcription Factor - genetics; STAT3 Transcription Factor - metabolism
• ISSN: 1433-6510
• DOI: 10.7754/Clin.Lab.2023.230917

miR-34a has been implicated in many autoimmune diseases and gastrointestinal diseases. However, the expression of miR-34 in ulcerative colitis (UC) patients were not fully studied. This study was performed to in-vestigate the association of blood and intestinal tissue miR-34a expression of patients with disease severity in UC patients. Our study enrolled 82 patients with UC and 80 age- and gender- matched healthy individuals. Blood miR-34a expressions were detected using reverse transcription-polymerase chain reaction (RT-PCR). Local intestinal miR-34a, STAT3 mRNA and IL-23 mRNA expressions were also detected in the lesioned area and adjacent non-affected intestinal tissue in patients. Disease severity of UC was assessed by Mayo score. The diagnostic value of both blood and local miR-34a expression for UC patients was assessed by receiver operating characteristic (ROC) curve. Blood miR-34a was increased in UC patients in contrast with healthy individuals with statistical significance. In UC patients, local intestinal miR-34a expressions were markedly upregulated compared to adjacent non-affected intestinal tissue. Local intestinal miR-34a expressions were positively correlated with STAT3 mRNA and IL-23 mNRA. Both blood and local miR-34a expressions were significantly and positively related to Mayo scores. ROC curve analysis indicated that both blood and local miR-34a expressions may act as decent marker for Mayo grade. Blood and intestinal tissue miR-34a expressions are correlated with disease severity in UC patients. Both blood and intestinal tissue miR-34a expressions may serve as potential diagnostic and prognostic makers for UC. Therapeutic methods targeting miR-34a may act as potential ways for UC treatment.