Impregnation of two-dimensional manganese dioxide nanosheets with 5-carboxyfluorescein as an immunoassay platform for sensitive detection of cardiac troponin T

• Published in: Mikrochimica acta (1966) Vol. 191; no. 11; p. 651
• Main Authors: Varghese, Susan, Madanan, Anju S., Abraham, Merin K., Shkhair, Ali Ibrahim, Indongo, Geneva, Rajeevan, Greeshma, K, Arathy B., George, Sony
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.11.2024; Springer Nature B.V
• Subjects: Analytical Chemistry; Biomolecules; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Fluoresceins - chemistry; Fluorescent Dyes - chemistry; Humans; Immunoassay; Immunoassay - methods; Limit of Detection; Manganese Compounds - chemistry; Manganese dioxide; Microengineering; Nanochemistry; Nanosheets; Nanostructures - chemistry; Nanotechnology; Oxides - chemistry; Troponin T - blood; Troponin T - immunology; Myocardial infarction; Immunoassay; Manganese dioxide nanosheet; Cardiac troponin T; 5-carboxyfluorescein
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-024-06727-0

A novel immunoassay platform is presented utilizing a cardiac troponin T antibody (Ab-cTnT) labelled with 5-carboxyfluorescein (5-FAM) integrated into a two-dimensional (2D) manganese dioxide nanosheet (MnO 2 NS) matrix. This strategy enables a turn-on response towards cTnT antigen within a mere 10-min incubation period, boasting an impressive lower detection limit of 0.038 ng/mL. Crucially, our probe demonstrates exceptional selectivity amidst the presence of coexisting biomolecules and ions, ensuring precise detection of cTnT. Moreover, the developed platform showcases promising utility in sensing cTnT from spiked human serum samples, yielding satisfactory recovery percentages ranging from 82 to 105%. Additionally, we introduce and easy-to-use and cost-effective test strip for point-of-care detection of cTnT, further enhancing accessibility to critical cardiovascular diagnostics. Graphical Abstract