Antimicrobial resistance, enterotoxin and biofilm production genes in Staphylococcus spp. isolated from facilities and fomites in veterinary hospital in the Caatinga biome

• Published in: Brazilian journal of microbiology Vol. 55; no. 3; pp. 2885 - 2892
• Main Authors: de Souto Sobrinho, José Diniz, de Valença Silva, Ana Karolione, de Medeiros, Katianny Bezerra, Silva, Maria Luana Cristiny Rodrigues, de Medeiros, Ana Beatriz Monteiro, de Sousa, Débora Luise Canuto, de Azevedo, Sérgio Santos, de Sousa Américo Batista Santos, Carolina
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2024; Springer Nature B.V
• Subjects: Animal diseases; Antimicrobial agents; Antimicrobial resistance; Biofilms; Biomedical and Life Sciences; Drug resistance; Ecosystems; Enterotoxins; Epidemiology; Fomites; Food Microbiology; Gene frequency; Genes; Hospital facilities; Hospitals; Infectious diseases; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Multidrug resistance; Mycology; Penicillin; Staphylococcus; Sterilization; Stethoscopes; Veterinary and Animal Production - Research Paper; Brazil; Nosocomial environment; gene; Bacterial AMR; One Health; SEE gene; icaD gene
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1007/s42770-024-01400-3

The Caatinga biome occurs only in Brazil and offers epidemiological conditions that should be assessed differently from other regions of Brazil and the world. Thus, the aim of this survey was to identify antimicrobial resistance, enterotoxin and biofilm production genes in Staphylococcus spp . isolated from facilities and fomites in a veterinary hospital in Caatinga biome. Samples were collected from surfaces of small animal clinical care tables ( n =8), cages in the dog and cat hospitalisation sector and animals with infectious diseases ( n = 21), small animal surgical centre ( n =8), sterilisation sector ( n =7) and stethoscopes ( n = 32) by using sterile swabs. Bacterial isolation and identification, antimicrobial resistance phenotypic test and molecular detection of antimicrobial resistance, biofilm formation and enterotoxin genes were carried out. Ninety-five bacterial isolates were obtained, and 29 (30.5%) were identified as Staphylococcus spp. Overall, 13 isolates (44.8%) of six species of Staphylococcus spp. showed antimicrobial resistance profile, as well as S. haemolyticus expressed phenotypic profile of multidrug resistance. The antimicrobials with the highest resistance rates were penicillin and tetracycline. The most frequent resistance genes were blaZ and tetM , both detected in 10 (76.9%) isolates. The mecA , tetL and tetK genes had frequencies of 38.5% (5/13), 23.1% (3/13) and 15.4% (2/13), respectively. The biofilm production marker, icaD gene, was detected in one S. sciuri strain. SEE gene, which encodes enterotoxins, was detected in 15.4% (2/13) of the strains ( S. pseudintermedius and S. intermedius ). The occurrence of Staphylococcus spp. carrying resistance genes to diferent classes of antimicrobials, presenting MDR phenotypic pattern and carrying enterotoxins and biofim encoding genes recovered from veterinary hospital facilities and fomites in the Caatinga biome reinforce the need to implement prevention cares in veterinary practices to avoid One Health-concerning conditions.