Purification and cDNA Cloning of Lysozyme Ⅱ from Cabbage Butterfly,Artogeia rapae Larvae

• Published in: Entomological research Vol. 35; no. 4; pp. 207 - 211
• Main Authors: Bang, I.S. (Dankook University, Cheonan, Republic of Korea), Yoe, S.M. (Dankook University, Cheonan, Republic of Korea), E-mail: smyoe@dankook.ac.kr
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2005; 한국곤충학회
• Subjects: Artogeia rapae; Insect immunity; LISOZIMA; LYSOZYME; Lysozyme gene; 생물학
• ISSN: 1738-2297; 1748-5967; 1748-5967
• DOI: 10.1111/j.1748-5967.2005.tb00161.x

Last instar larvae of cabbage butterfly Artogeia rapae respond to injection of bacteria with a set of inducible antibacterial peptides/proteins. The inducible peptides/proteins are related to the known hinnavins (Ⅰ and Ⅱ) and lysozymes (Ⅰ and Ⅱ). The lysozyme Ⅱ has been isolated by heat treatment, cation exchange, and reversed-phase chromatography from immunized hemolymph of last instar larvae. The lysozyme Ⅱ gene of A. rapae was isolated and its nucleotide sequence was determined by the RACE-PCR from immunized fat body with E. coli. It has an open reading frame of 414 bp nucleotide corresponding to 138 amino acids including an 18 amino acid signal sequence.