Alcohol dehydrogenase controls the flux from ethanol into lipids in Drosophila larvae. A 13C NMR study
The dependence of the flux in the alcohol-degrading pathway on the activity of alcohol dehydrogenase was investigated in Drosophila larvae. Third-instar larvae were supplied with [2-13C]ethanol as a dietary carbon source. Specific carbon enrichments in de novo synthesized fatty acids were determined...
Saved in:
Published in | The Journal of biological chemistry Vol. 266; no. 32; pp. 21399 - 21403 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
15.11.1991
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The dependence of the flux in the alcohol-degrading pathway on the activity of alcohol dehydrogenase was investigated in Drosophila
larvae. Third-instar larvae were supplied with [2-13C]ethanol as a dietary carbon source. Specific carbon enrichments in de
novo synthesized fatty acids were determined in vitro by means of 13C nuclear magnetic resonance spectroscopy. Carbon fluxes
deduced from these enrichment patterns were correlated with the in vitro alcohol dehydrogenase activities in three different
Adh genotypes in seven different strains. The flux control coefficient for alcohol dehydrogenase was shown to be approximately
1.0. This indicates that the alcohol dehydrogenase gene-enzyme system in Drosophila larvae can be a major target of natural
selection. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)54651-2 |