Isolation and culture of murine heart and lung endothelial cells for in vitro model systems

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 341; p. 141
• Main Authors: Lim, Yaw-Chyn, Luscinskas, Francis W
• Format: Journal Article
• Language: English
• Published: United States 2006
• Subjects: Animals; Cell Adhesion; Cell Fractionation - methods; Cell Movement; Cells, Cultured; Endothelial Cells - cytology; Endothelial Cells - metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Inflammation - metabolism; Leukocytes - cytology; Leukocytes - metabolism; Lung - blood supply; Lung - cytology; Lung - metabolism; Mice; Models, Biological; Myocardium - cytology; Myocardium - metabolism
• ISSN: 1064-3745
• DOI: 10.1385/1-59745-113-4:141

The inflammatory response is a critical component of host defense. An important goal of our group has been to understand the endothelial-dependent mechanisms that mediate leukocyte recruitment during an inflammatory response. In this chapter, we present a detailed method for the isolation and in vitro culture of murine vascular endothelial cells from the lung and heart. The endothelial cells are of high purity (85-99%) and retain certain of their functional differences, including constitutive and cytokine inducible adhesion molecule expression and chemokine production. Such endothelial cells, therefore, can be used for various in vitro models including leukocyte adhesion assay or in depth biochemical analyses.