Intensity of apoptotic processes, aconitate hydratase activity and citrate level in patients with type 2 diabetes mellitus complicated steatohepatitis under application of epifamin at basic therapy

• Published in: Biomeditsinskaia khimiia Vol. 61; no. 3; p. 400
• Main Authors: Popov, S S, Pashkov, A N, Agarkov, A A, Shulgin, K K
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.2015
• Subjects: Aconitate Hydratase - blood; Aged; Aged, 80 and over; Apoptosis - drug effects; Caspase 3 - blood; Citric Acid - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - drug therapy; Female; Humans; Male; Middle Aged; Non-alcoholic Fatty Liver Disease - drug therapy; Non-alcoholic Fatty Liver Disease - etiology; Peptides - pharmacology; epifamin; steatohepatitis; citrate; patients; type 2 diabetes mellitus; apoptosis; free-radical oxidation; caspases; aconitate hydratase
• ISSN: 2310-6972
• DOI: 10.18097/PBMC20156103400

DNA fragmentation, caspase-1 and caspase-3, aconitate hydratase (AH) activities, and citrate content have been investigated in the blood of patients with type 2 diabetes mellitus complicated by steatohepatitis. These indicators of apoptotic processes intensity and oxidative stress development were estimated after basic treatment and a combined therapy including epifamin. Before treatment DNA fragmentation blood leukocytes, decrease of AH activity and increase of caspases activities in the serum of patients were detected. Treatment with epifamin provided more pronounced changes in the investigated parameters towards control values as compared to basis therapy. Epifamin caused a positive effect on the citrate content in the serum of patients. Epifamin inclusion to the basic therapy was accompanied by a more pronounced changes towards the normal values of such biochemical parameters as ALT, AST, b-lipoproteins, cholesterol, fasting glucose and postprandial glucose levels. All these changes may be obviously attributed to epifamin-induced correction of the melatonin level and manifestation of adaptogenic properties and antioxidant effects of the hormone.