Freely Available Tool (FAT) for automated quantification of lipid droplets in stained cells

In this study, we propose an automatic procedure for digital image processing. We describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in culture. Prospectively, the lipid droplets detection method described in this work could be appli...

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Bibliographic Details
Published inBiocell Vol. 41; no. 2; pp. 55 - 58
Main Authors Masone, Diego, D. Gojanovich, Aldana, R. Frontini-Lopez, Yesica, DEL Veliz, Samanta, Uhart, Marina, M. Bustos, Diego
Format Journal Article
LanguageEnglish
Published Mendoza Tech Science Press 2017
Subjects
Automation
Cell culture
Fluorescence microscopy
Image processing
Lipids
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Summary:In this study, we propose an automatic procedure for digital image processing. We describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in culture. Prospectively, the lipid droplets detection method described in this work could be applied to static or time-lapse data, collected with a simple visible light or fluorescence microscopy equipment. Fully automated algorithms were implemented in Octave, a freely available scientific package.
ISSN:1667-5746
0327-9545
DOI:10.32604/biocell.2017.41.055