The anti-cancer impact of genistein against acute lymphoblastic leukaemia by controlling DICER and AGO2 involved in cytoplasmic microRNAs biogenesis—a possible new clue to mode of action of genistein
Childhood acute lymphoblastic leukaemia (ALL) is one of the most prevalent malignancies. The alteration in expression of the ibonuclease III is a Protein Coding gene (DICER) and Argonaute RISC Catalytic Component 2 (AGO2) was assessed in human samples and four ALL cell lines. Then, the inhibitory ef...
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Published in | Journal of herbal medicine Vol. 41; p. 100731 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier GmbH
01.09.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Childhood acute lymphoblastic leukaemia (ALL) is one of the most prevalent malignancies.
The alteration in expression of the ibonuclease III is a Protein Coding gene (DICER) and Argonaute RISC Catalytic Component 2 (AGO2) was assessed in human samples and four ALL cell lines. Then, the inhibitory effect of genistein on ALL cell lines was evaluated.
Up-regulation of DICER and down-regulation of AGO2 were observed in 40 patients with ALL compared to 35 healthy controls. The comparison of their alteration among different stages revealed their role in the progression of ALL. Genistein had dose- and time-dependent anti-proliferative effects against ALL cancer cells through inducing apoptosis and decreasing the growth rate of malignant cells. Genistein significantly elevated the level of DICER, particularly in two cells (Molt-17 and Nalm-6). The up regulation of AGO2 was significant after genistein treatment in the four cell lines as compared to non-treated cells.
Alteration in the expression of these genes is correlated with the progression of the disease. The alteration of AGO2 and DICER expressions in B-ALL cell lines after genistein treatment provide this evidence to support our hypothesis that genistein can inhibit the growth of cancerous cells, particularly ALL cells, by mediating changes to elements that contribute to the biogenesis of endogenous microRNAs, such as AGO2 and DICER, thus affecting different cellular pathways in cancerous cells. This study seems to have clarified another anti-cancerous mechanism of genistein, which can be promising. |
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ISSN: | 2210-8033 |
DOI: | 10.1016/j.hermed.2023.100731 |