Application of magnetic nanomaterials in peptidomics: A review in the past decade

• Published in: Chinese chemical letters Vol. 36; no. 6; p. 110070
• Main Authors: Guo, Yimin, Luo, Yiting, Hua, Shuwen, Ding, Chuan-Fan, Yan, Yinghua
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2025
• Subjects: Enrichment; Glycosylation; Magnetic nanomaterials; Mass spectrometry; Phosphorylation; Enrichment; Phosphorylation; Glycosylation; Mass spectrometry; Magnetic nanomaterials
• ISSN: 1001-8417
• DOI: 10.1016/j.cclet.2024.110070

Protein glycosylation and phosphorylation, as two of the most important protein post-translational modifications (PTMs), play key roles in living organisms. However, glycopeptides and phosphopeptides have low abundance in biological samples. In addition, the low ionization efficiency and the severe signal interference in the presence of other peptides present great difficulties for their direct mass spectrometry (MS) analysis. Therefore, it is important to develop feasible enrichment strategies to pretreat glycopeptides and phosphopeptides in complex samples before MS detection. This paper reviews the application of various magnetic nanomaterials (MNMs) in glycopeptides and phosphopeptides in the last decade, with emphasis on the enrichment principles, the design and synthesis process of the materials, and the effectiveness of the application in biological samples. In addition, possible future trends and potential challenges are presented. [Display omitted] This review mainly focuses on the research progress of magnetic nanomaterials (MNMs) in peptidomics in the last decade, including both standard and complex biological samples.