Elucidating the physicochemical and structural properties of Ganoderma lucidum spores: Comparative analysis of various disruption techniques

This study evaluated Ganoderma lucidum G2 spores (GLS) using various processing techniques: vibrating milling, ball milling, and autoclaving. Broken GLS via vibrating and ball milling showed higher lipid content (22.89% and 22.50%) but lower L*, a*, and b* values compared to intact GLS. Vibrating mi...

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Published inPowder technology Vol. 439; p. 119731
Main Authors Sriket, Chodsana, Kuimalee, Surasak, Yarnpakdee, Suthasinee, Benjakul, Soottawat, Sriket, Pornpimol, Kishimura, Hideki, Senphan, Theeraphol, Nalinanon, Sitthipong
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.04.2024
Subjects
Ganoderma lucidum
Physicochemical quality
Spore-breaking
Structural quality
Various techniques
Physicochemical quality
Ganoderma lucidum
Structural quality
Spore-breaking
Various techniques
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Summary:This study evaluated Ganoderma lucidum G2 spores (GLS) using various processing techniques: vibrating milling, ball milling, and autoclaving. Broken GLS via vibrating and ball milling showed higher lipid content (22.89% and 22.50%) but lower L*, a*, and b* values compared to intact GLS. Vibrating milling achieved the highest sporoderm-broken rate (98.27%). Ball milling resulted in the highest lipid oxidation. Vibrating milling yielded the highest bioactive compounds, and both methods increased DPPH-RSA values. Differential scanning calorimetry indicated varying Tmax values: non-broken GLS (106.56 °C), vibrating milling (92.77 °C), ball milling (97.58 °C), and autoclaving (109.35 °C). FT-IR spectra showed triterpenes, polysaccharides, and fatty acids. X-ray diffraction and Fourier transform infrared spectroscopy revealed increased crystallinity with vibrating milling (37.12% to 47.99%). Both milling methods altered GLS structure to a disintegrated, pancake-like form. Scanning electron microscopy with energy-dispersive X-ray spectroscopy indicated significant carbon and oxygen concentrations. This study provided crucial insights for utilizing GLS. [Display omitted] •Vibrating and ball milling boost Ganoderma lucidum spore (GLS)’ lipid content.•FTIR identifies triterpenes, polysaccharides and fatty acids in treated GLS spores.•Vibrating milling elevates crystallinity from 37.12% to 47.99% in GLS.•Vibrating and ball milling reshape GLS from pear-shaped to pancake-like structures.•Important findings inform GLS's future development and application.
ISSN:0032-5910
1873-328X
DOI:10.1016/j.powtec.2024.119731