Multi-signal logic-gate sensing platform based on CoOOH nanoflakes for quantitative point-of-care detection of prostate specific antigen
[Display omitted] A cobalt oxyhydroxide (CoOOH) nanoflake-mediated multi-signal sensing system for the immediate detection of biomarkers is proposed. The NADH of the signal probe inhibited the peroxidase-like activity of the CoOOH nanosheets, leading to decreased pressure, weight and temperature res...
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Published in | Microchemical journal Vol. 194; p. 109299 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.11.2023
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
A cobalt oxyhydroxide (CoOOH) nanoflake-mediated multi-signal sensing system for the immediate detection of biomarkers is proposed. The NADH of the signal probe inhibited the peroxidase-like activity of the CoOOH nanosheets, leading to decreased pressure, weight and temperature responses in the sealed system, in line with the “INHIBIT” logic gate.
•A multi-signal mode with sensitivity does not require any expensive or bulky analytical equipment or laborious operation.•This approach can be regarded as a versatile tool for monitoring other biomarkers by replacing the antibody.•We combined the concept of the “INHIBIT” logic gate with POCT.
Herein, we propose a cobalt oxyhydroxide (CoOOH) nanoflake-mediated multi-signal sensing system for the immediate detection of biomarkers. The platform employs monoclonal antibody Ab1-modified magnetic nanoparticles (CuFe2O4-Ab1) as the capture target and multiwalled carbon nanotubes (MWCNTs) modified with polyclonal antibody Ab2 and nicotinamide adenine dinucleotide (NADH) as the signal probe (Ab2-MWCNTs-APBA-NADH). By taking the prostate-specific antigen (PSA) as a target, the feasibility of the sensing platform was confirmed. When exposed to PSA, the sandwich immune complex (CuFe2O4-Ab1/PSA/Ab2-MWCNTs-APBA-NADH) was formed by antigen–antibody specific recognition. Thereafter, the NADH of the signal probe inhibited the peroxidase-like activity of the CoOOH nanosheets, leading to decreased pressure, weight and temperature responses in the sealed system, in line with the “INHIBIT” logic gate. The three responses had a strong linear relationship with PSA concentration, with relative standard deviations (RSD) of 0.1–1.5%. With simple procedures and straightforward readout strategies, the proposed method can be easily generalized for specific detection of other analytes by changing the antibodies. |
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ISSN: | 0026-265X 1095-9149 |
DOI: | 10.1016/j.microc.2023.109299 |