Multi-signal logic-gate sensing platform based on CoOOH nanoflakes for quantitative point-of-care detection of prostate specific antigen

• Published in: Microchemical journal Vol. 194; p. 109299
• Main Authors: Tan, Yunzhu, Gao, Rong, Wen, Anlin, Liu, Bingqian
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2023
• Subjects: Cobalt oxyhydroxide nanoflakes (CoOOH); Logic gate; Multi-signal sensing platform; POCT; PSA; Logic gate; Multi-signal sensing platform; POCT; Cobalt oxyhydroxide nanoflakes (CoOOH); PSA
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2023.109299

[Display omitted] A cobalt oxyhydroxide (CoOOH) nanoflake-mediated multi-signal sensing system for the immediate detection of biomarkers is proposed. The NADH of the signal probe inhibited the peroxidase-like activity of the CoOOH nanosheets, leading to decreased pressure, weight and temperature responses in the sealed system, in line with the “INHIBIT” logic gate. •A multi-signal mode with sensitivity does not require any expensive or bulky analytical equipment or laborious operation.•This approach can be regarded as a versatile tool for monitoring other biomarkers by replacing the antibody.•We combined the concept of the “INHIBIT” logic gate with POCT. Herein, we propose a cobalt oxyhydroxide (CoOOH) nanoflake-mediated multi-signal sensing system for the immediate detection of biomarkers. The platform employs monoclonal antibody Ab1-modified magnetic nanoparticles (CuFe2O4-Ab1) as the capture target and multiwalled carbon nanotubes (MWCNTs) modified with polyclonal antibody Ab2 and nicotinamide adenine dinucleotide (NADH) as the signal probe (Ab2-MWCNTs-APBA-NADH). By taking the prostate-specific antigen (PSA) as a target, the feasibility of the sensing platform was confirmed. When exposed to PSA, the sandwich immune complex (CuFe2O4-Ab1/PSA/Ab2-MWCNTs-APBA-NADH) was formed by antigen–antibody specific recognition. Thereafter, the NADH of the signal probe inhibited the peroxidase-like activity of the CoOOH nanosheets, leading to decreased pressure, weight and temperature responses in the sealed system, in line with the “INHIBIT” logic gate. The three responses had a strong linear relationship with PSA concentration, with relative standard deviations (RSD) of 0.1–1.5%. With simple procedures and straightforward readout strategies, the proposed method can be easily generalized for specific detection of other analytes by changing the antibodies.