Proteomic approaches for generating comprehensive protein interaction maps

• Published in: Targets Vol. 2; no. 3; pp. 85 - 92
• Main Authors: Auerbach, Daniel, Fetchko, Michael, Stagljar, Igor
• Format: Book Review Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2003
• Subjects: mass spectrometry; protein interaction maps; protein interactions; proteome chips; proteomics; yeast two-hybrid; protein interactions; proteome chips; proteomics; Techniques & Methods; Pharmaceutical Science; yeast two-hybrid; mass spectrometry; Genetics; Biochemistry; protein interaction maps; Drug Discovery
• ISSN: 1477-3627; 1878-0989
• DOI: 10.1016/S1477-3627(03)02316-X

The availability of complete genome sequences of numerous model organisms has initiated the development of new approaches in biological research to complement conventional biochemistry and genetics. In this context, high-throughput methods for detecting protein interactions, such as mass spectrometry and yeast two-hybrid assays, have produced vast amounts of data that can be exploited to infer protein function and regulation. In this review, we explore different genome-wide protein interaction studies and comment on their extrapolation towards understanding protein functions. It is likely that improvements of these approaches, together with more sophisticated databases and the invention of novel technologies, will help to decipher the complex interactions among proteins and to integrate interacting proteins into existing and novel cellular pathways.