Production of Recombinant Hinnavin Ⅱ/MSH Hybrid in Insect Cells

The increasing problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. For the purpose of this study, a cDNA encoding hinnavin Ⅱ-α-melanocyte stimulating hormone (hin/MSH) hybrid was chemically synthesized, annealed, and then cloned into transfer...

Full description

Saved in:
Bibliographic Details
Published inEntomological research Vol. 35; no. 1; pp. 27 - 31
Main Authors Bang, I.S. (Hoseo University, Asan, Republic of Korea), Park, S.H. (Hoseo University, Asan, Republic of Korea), Kang, C.S. (Hoseo University, Asan, Republic of Korea), Bang, S.K. (Hoseo University, Asan, Republic of Korea), E-mail: skbang@office.hoseo.ac.kr
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.03.2005
한국곤충학회
Subjects
Antimicrobial peptide
ANTIMICROBIALS
ANTIMICROBIANOS
ANTIMICROBIEN
BEVS
Hin/MSH hybrid
Hinnavin
Hinnavin II
MSH
생물학
Online AccessGet full text

Cover

More Information
Summary:The increasing problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. For the purpose of this study, a cDNA encoding hinnavin Ⅱ-α-melanocyte stimulating hormone (hin/MSH) hybrid was chemically synthesized, annealed, and then cloned into transfer vector pBacPAK 9 for expression in Sf21 insect cells. Recombinant hin/MSH (rhin/MSH) hybrid was efficiently produced in baculovirus expression vector system (BEVS) as a hybrid peptide. The antibacterial activity of the rhin/MSH hybrid was compared to that of the recombinant hinnavin Ⅱ (rhin), using inhibition zone and overlay assay.
Bibliography:2006016108
H10
istex:B7495CFA0BE4BC89E42940F4937FFA2870C31305
ArticleID:ENR27
ark:/67375/WNG-MFMRR969-P
G704-000624.2005.35.1.006
ISSN:1738-2297
1748-5967
1748-5967
DOI:10.1111/j.1748-5967.2005.tb00133.x