Interaction of POPG membranes with ionic liquids containing 1-Dodecyl-3-methylbenzimidazolium and 1-Dodecyl-1-methylmorpholinium Cations: Structural details from 31P and 2H-based solid-state NMR spectroscopy

• Published in: Journal of Magnetic Resonance Open Vol. 10-11; p. 100036
• Main Authors: Kaur, Navleen, Fischer, Markus, Kumar, Sandeep, Gahlay, Gagandeep Kaur, Scheidt, Holger A., Mithu, Venus Singh
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.06.2022; Elsevier
• Subjects: 2H and 31P nuclear magnetic resonance, solid-state NMR spectroscopy; Amphiphilic cations; Ionic liquids; Membrane structure and dynamics; Ionic liquids; 2H and 31P nuclear magnetic resonance, solid-state NMR spectroscopy; Membrane structure and dynamics; Amphiphilic cations
• ISSN: 2666-4410; 2666-4410
• DOI: 10.1016/j.jmro.2022.100036

•Benzimidazolium, morpholinium, and imidazolium-based ionic liquids are compared.•Benzimidazolium is most toxic and membrane permeabilizing; morpholinium the least.•31P and 2H-based ssNMR spectroscopy reveals structural details of interaction with POPG membranes.•31P CSA of POPG is least perturbed; membrane head-group region is not affected.•1-Dodecyl-3-methylbenzimidazolium cations make POPG chains more disordered; can cause membrane fusion. Interactions of ionic liquids (ILs) with phospholipid membranes play a crucial role in their biological activity be it their cytotoxicity or their application as drug delivery agents. To develop the design principles for task-specific applications of ionic liquids, structural details of this interaction with atomic resolution must be obtained. In this context, 31P and 2H-based solid-state NMR spectroscopy of phospholipid membranes is a very useful technique. Here, we have used it to study the impact of benzimidazolium (BNZ) and morpholinium (MPH) cation-based ionic liquids on the head-group orientation and acyl chain disorder of negatively charged POPG membranes. Owing to the large-size and aromatic nature of its head group, membrane partitioning of BNZ cations increases the disorder of the entire POPG acyl chain. Comparisons are drawn with the behavior of widely studied imidazolium (IMI) cation which, like MPH, has a contrasting impact in the upper and lower halves of POPG chains. The observations are used to rationalize the higher potential of BNZ to permeabilize and fuse POPG vesicles, which was determined using fluorescence-based dye leakage and lipid mixing assays. [Display omitted]