3D Endothelial Cell Migration

Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this vessel sprout. Here, we describe...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1749; p. 51
Main Authors Jacobs, Kathryn A, Gavard, Julie
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Cell Line, Tumor
Cell Movement - physiology
Culture Media, Conditioned
Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells
Humans
Models, Biological
Neovascularization, Physiologic - physiology
Vascular Endothelial Growth Factor A - metabolism
Fibrin matrix
Endothelial cell
Sprouting angiogenesis
Conditioned medium
Tumor microenvironment
VEGF
Online AccessGet more information

Cover

More Information
Summary:Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this vessel sprout. Here, we describe a method to study in vitro this early and rapid stage of sprouting angiogenesis.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7701-7_6